Transfer and scale-up from 10 L BioBLU®️ to Allegro™ STR 50 and STR 200 Bioreactors

Adeno-associated viruses (AAV) are established vectors for delivering gene-therapy payloads, however the cost of goods transfection reagents and DNA, along with challenges to scaling-up process, presents obstacles industrialization recombinant adeno-associated virus (rAAV) therapeutic products overcome. The ELEVECTA® platform from CEVEC is a stable cell line inducible AAV production which removes need costly transient transfection. In this work we show that vector an producer scalable bench-scale 10 L bioreactors 50 200 using Pall AllegroTM STR STR paves way up 2000 Allegro bioreactor. We also demonstrate good capacity, yield scalability initial unit operations downstream harvest clarification tangential flow filtration.